User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/12: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==mm/dd/yy==
==12/12/12==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* U2OS: split cells (passage 3)
* SK-N-SH: split cells (passage 3) and set up transfection plate
 


----
----
'''Line item 1'''<br>
'''U2OS'''<br>
> Samples
* Split cells 1:10, 2x 75 cm flasks


{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
|-valign="top"
| <u>Reagent</u> || <u>Volume</u>
|-
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}


--> Reaction conditions
----
'''SK-N-SH'''
* Split cells 1:5, 1x 75 cm flask
* Seed 6-well plate for transfection, ~5x10<sup>5</sup> cells/well/1 mL
** Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well




Latest revision as of 22:19, 26 September 2017

Pc-TF Genomics Main project page
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12/12/12

  • U2OS: split cells (passage 3)
  • SK-N-SH: split cells (passage 3) and set up transfection plate


U2OS

  • Split cells 1:10, 2x 75 cm flasks


SK-N-SH

  • Split cells 1:5, 1x 75 cm flask
  • Seed 6-well plate for transfection, ~5x105 cells/well/1 mL
    • Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well



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