User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/12: Difference between revisions
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* | * U2OS: split cells (passage 3) | ||
* SK-N-SH: split cells (passage 3) and set up transfection plate | |||
---- | ---- | ||
''' | '''U2OS'''<br> | ||
* Split cells 1:10, 2x 75 cm flasks | |||
--> | ---- | ||
'''SK-N-SH''' | |||
* Split cells 1:5, 1x 75 cm flask | |||
* Seed 6-well plate for transfection, ~5x10<sup>5</sup> cells/well/1 mL | |||
** Added 3 mL of cells from 10 mL stock flask to 7 mL media; aliquoted 1 mL diluted cells to each well | |||
Revision as of 11:21, 13 December 2012
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12/12/12
U2OS
SK-N-SH
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