User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(→mm/dd/yy)
(→mm/dd/yy)
Line 8: Line 8:
-
==mm/dd/yy==
+
==12/18/12==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C
* TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C

Revision as of 18:05, 18 December 2012

Pc-TF Genomics Main project page
Previous entry      Next entry


12/18/12

  • TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C

TriZOL-RNeasy prep

  1. "K562 1": 1:2 dilution cells, +DNA
  2. "K562 1": 1:2 dilution cells, no DNA
  3. "K562 1": 1:4 dilution cells, +DNA
  4. "K562 1": 1:4 dilution cells, no DNA
  5. "K562 1": 1:8 dilution cells, +DNA
  6. "K562 1": 1:8 dilution cells, no DNA
  • Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
  • Pellet cells at r.t./ 3 min./ 2000 rpm
  • Discard supernatant
  • Resuspend pellet in 500 μL TriZOL
  • Store at -80°C for next steps (Carly)



Retrieved from "http://www.openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2012/12/18"
Personal tools