User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18

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* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
-
* Pellet cells at r.t./ 3 min./ 2000 rpm
+
* Pellet cells at r.t./ 5 min./ 3000 rpm
* Discard supernatant
* Discard supernatant
* Resuspend pellet in 500 μL TRIzol
* Resuspend pellet in 500 μL TRIzol

Revision as of 17:08, 18 December 2012

Pc-TF Genomics Main project page
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12/18/12

  • TRIzol-RNeasy prep: lyse cells in TriZOL; store at -80°C

TRIzol-RNeasy prep

  1. "K562 1": 1:2 dilution cells, +DNA
  2. "K562 1": 1:2 dilution cells, no DNA
  3. "K562 1": 1:4 dilution cells, +DNA
  4. "K562 1": 1:4 dilution cells, no DNA
  5. "K562 1": 1:8 dilution cells, +DNA
  6. "K562 1": 1:8 dilution cells, no DNA
  • Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
  • Pellet cells at r.t./ 5 min./ 3000 rpm
  • Discard supernatant
  • Resuspend pellet in 500 μL TRIzol
  • Store at -80°C for next steps (Carly)



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