User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18

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(12/18/12)
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==mm/dd/yy==
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==12/18/12==
<!-- Precede finished items with a checkmark &#x2713; -->
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* Line item 1
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* TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C
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'''Line item 1'''<br>
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'''TRIzol-RNeasy prep'''<br>
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> Samples
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# "K562 1": 1:2 dilution cells, +DNA
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# "K562 1": 1:2 dilution cells, no DNA
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
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# "K562 1": 1:4 dilution cells, +DNA
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|-valign="top"
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# "K562 1": 1:4 dilution cells, no DNA
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| <u>Reagent</u> || <u>Volume</u>
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# "K562 1": 1:8 dilution cells, +DNA
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|-
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# "K562 1": 1:8 dilution cells, no DNA
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| reagent 1 || # μL
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|-
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| reagent 2 || #
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|-
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| reagent 3 || #
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|-
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| reagent 4 || #
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|-
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| dH<sub>2</sub>O || #
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|-
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| &nbsp; || # μL
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|}
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--> Reaction conditions
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* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
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* Pellet cells at r.t./ 5 min./ 3000 rpm
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* Discard supernatant
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* Resuspend pellet in 500 μL TRIzol
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* Store at -80°C for next steps (Carly)

Revision as of 18:34, 18 December 2012

Pc-TF Genomics Main project page
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12/18/12

  • TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C



TRIzol-RNeasy prep

  1. "K562 1": 1:2 dilution cells, +DNA
  2. "K562 1": 1:2 dilution cells, no DNA
  3. "K562 1": 1:4 dilution cells, +DNA
  4. "K562 1": 1:4 dilution cells, no DNA
  5. "K562 1": 1:8 dilution cells, +DNA
  6. "K562 1": 1:8 dilution cells, no DNA
  • Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
  • Pellet cells at r.t./ 5 min./ 3000 rpm
  • Discard supernatant
  • Resuspend pellet in 500 μL TRIzol
  • Store at -80°C for next steps (Carly)



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