User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18

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==mm/dd/yy==
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==12/18/12==
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* TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C
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* TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C
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'''TriZOL-RNeasy prep'''<br>
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'''TRIzol-RNeasy prep'''<br>
# "K562 1": 1:2 dilution cells, +DNA
# "K562 1": 1:2 dilution cells, +DNA
# "K562 1": 1:2 dilution cells, no DNA
# "K562 1": 1:2 dilution cells, no DNA
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* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
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* Pellet cells at r.t./ 3 min./ 2000 rpm
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* Pellet cells at r.t./ 5 min./ 3000 rpm
* Discard supernatant
* Discard supernatant
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* Resuspend pellet in 500 μL TriZOL
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* Resuspend pellet in 500 μL TRIzol
* Store at -80°C for next steps (Carly)
* Store at -80°C for next steps (Carly)

Revision as of 18:34, 18 December 2012

Pc-TF Genomics Main project page
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12/18/12

  • TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C



TRIzol-RNeasy prep

  1. "K562 1": 1:2 dilution cells, +DNA
  2. "K562 1": 1:2 dilution cells, no DNA
  3. "K562 1": 1:4 dilution cells, +DNA
  4. "K562 1": 1:4 dilution cells, no DNA
  5. "K562 1": 1:8 dilution cells, +DNA
  6. "K562 1": 1:8 dilution cells, no DNA
  • Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
  • Pellet cells at r.t./ 5 min./ 3000 rpm
  • Discard supernatant
  • Resuspend pellet in 500 μL TRIzol
  • Store at -80°C for next steps (Carly)



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