User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18: Difference between revisions
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==12/18/12== | ==12/18/12== | ||
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* TRIzol-RNeasy prep: lyse cells in | * TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C | ||
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* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture | * Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture | ||
* Pellet cells at r.t./ | * Pellet cells at r.t./ 5 min./ 3000 rpm | ||
* Discard supernatant | * Discard supernatant | ||
* Resuspend pellet in 500 μL TRIzol | * Resuspend pellet in 500 μL TRIzol |
Revision as of 15:34, 18 December 2012
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12/18/12
TRIzol-RNeasy prep
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