User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Pc-TF Genomics</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==12/18/12== | ||
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* | * TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C | ||
---- | ---- | ||
''' | '''TRIzol-RNeasy prep'''<br> | ||
# "K562 1": 1:2 dilution cells, +DNA | |||
# "K562 1": 1:2 dilution cells, no DNA | |||
# "K562 1": 1:4 dilution cells, +DNA | |||
# "K562 1": 1:4 dilution cells, no DNA | |||
# "K562 1": 1:8 dilution cells, +DNA | |||
# "K562 1": 1:8 dilution cells, no DNA | |||
* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (did this 4x for each culture); discard remainder of culture | |||
* Pellet cells at r.t./ 5 min./ 3000 rpm | |||
* Discard supernatant | |||
* Resuspend pellet in 500 μL TRIzol | |||
* Store at -80°C for next steps (Carly) | |||
Latest revision as of 22:20, 26 September 2017
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12/18/12
TRIzol-RNeasy prep
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