User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18: Difference between revisions
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==mm/dd/yy== | ==mm/dd/yy== | ||
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* | * TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C | ||
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''' | '''TriZOL-RNeasy prep'''<br> | ||
# "K562 1": 1:2 dilution cells, +DNA | |||
# "K562 1": 1:2 dilution cells, no DNA | |||
# "K562 1": 1:4 dilution cells, +DNA | |||
# "K562 1": 1:4 dilution cells, no DNA | |||
# "K562 1": 1:8 dilution cells, +DNA | |||
# "K562 1": 1:8 dilution cells, no DNA | |||
* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture | |||
* Pellet cells at r.t./ 3 min./ 2000 rpm | |||
* Discard supernatant | |||
* Resuspend pellet in 500 μL TriZOL | |||
* Store at -80°C for next steps (Carly) | |||
Revision as of 15:05, 18 December 2012
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mm/dd/yy
TriZOL-RNeasy prep
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