User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/18 - Revision history

Karmella Haynes: /* 12/18/12 */

Karmella Haynes — Tue, 18 Dec 2012 22:34:23 GMT

12/18/12

←Older revision		Revision as of 22:34, 18 December 2012
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	==12/18/12==		==12/18/12==
	<!-- Precede finished items with a checkmark ✓ -->		<!-- Precede finished items with a checkmark ✓ -->
-	* TRIzol-RNeasy prep: lyse cells in ~~TriZOL~~; store at -80°C	+	* TRIzol-RNeasy prep: lyse cells in TRIzol; store at -80°C

Karmella Haynes: /* 12/18/12 */

Karmella Haynes — Tue, 18 Dec 2012 22:34:07 GMT

12/18/12

←Older revision		Revision as of 22:34, 18 December 2012
Line 11:		Line 11:
	<!-- Precede finished items with a checkmark ✓ -->		<!-- Precede finished items with a checkmark ✓ -->
	* TRIzol-RNeasy prep: lyse cells in TriZOL; store at -80°C		* TRIzol-RNeasy prep: lyse cells in TriZOL; store at -80°C
		+

	----		----

Karmella Haynes: /* 12/18/12 */

Karmella Haynes — Tue, 18 Dec 2012 22:08:33 GMT

12/18/12

←Older revision		Revision as of 22:08, 18 December 2012
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	* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture		* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
-	* Pellet cells at r.t./ 3 min./ ~~2000~~ rpm	+	* Pellet cells at r.t./ 5 min./ 3000 rpm
	* Discard supernatant		* Discard supernatant
	* Resuspend pellet in 500 μL TRIzol		* Resuspend pellet in 500 μL TRIzol

Karmella Haynes: /* 12/18/12 */

Karmella Haynes — Tue, 18 Dec 2012 22:06:40 GMT

12/18/12

←Older revision		Revision as of 22:06, 18 December 2012
Line 10:		Line 10:
	==12/18/12==		==12/18/12==
	<!-- Precede finished items with a checkmark ✓ -->		<!-- Precede finished items with a checkmark ✓ -->
-	* ~~TriZOL~~-RNeasy prep: lyse cells in TriZOL; store at -80°C	+	* TRIzol-RNeasy prep: lyse cells in TriZOL; store at -80°C

	----		----
-	'''~~TriZOL~~-RNeasy prep'''<br>	+	'''TRIzol-RNeasy prep'''<br>
	# "K562 1": 1:2 dilution cells, +DNA		# "K562 1": 1:2 dilution cells, +DNA
	# "K562 1": 1:2 dilution cells, no DNA		# "K562 1": 1:2 dilution cells, no DNA
Line 24:		Line 24:
	* Pellet cells at r.t./ 3 min./ 2000 rpm		* Pellet cells at r.t./ 3 min./ 2000 rpm
	* Discard supernatant		* Discard supernatant
-	* Resuspend pellet in 500 μL ~~TriZOL~~	+	* Resuspend pellet in 500 μL TRIzol
	* Store at -80°C for next steps (Carly)		* Store at -80°C for next steps (Carly)

Karmella Haynes: /* mm/dd/yy */

Karmella Haynes — Tue, 18 Dec 2012 22:05:55 GMT

mm/dd/yy

←Older revision		Revision as of 22:05, 18 December 2012
Line 8:		Line 8:


-	==mm/dd/yy==	+	==12/18/12==
	<!-- Precede finished items with a checkmark ✓ -->		<!-- Precede finished items with a checkmark ✓ -->
	* TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C		* TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C

Karmella Haynes: /* mm/dd/yy */

Karmella Haynes — Tue, 18 Dec 2012 22:05:37 GMT

mm/dd/yy

←Older revision		Revision as of 22:05, 18 December 2012
Line 10:		Line 10:
	==mm/dd/yy==		==mm/dd/yy==
	<!-- Precede finished items with a checkmark ✓ -->		<!-- Precede finished items with a checkmark ✓ -->
-	* ~~Line item 1~~	+	* TriZOL-RNeasy prep: lyse cells in TriZOL; store at -80°C

	----		----
-	'''~~Line item 1~~'''<br>	+	'''TriZOL-RNeasy prep'''<br>
-	~~> Samples~~	+	# "K562 1": 1:2 dilution cells, +DNA
-		+	# "K562 1": 1:2 dilution cells, no DNA
-	~~{\| class=~~"~~wikitable~~" ~~border=~~"0" ~~cellspacing=~~"3" ~~<!-- Rxn. table -->~~	+	# "K562 1": 1:4 dilution cells, +DNA
-	~~\|-valign=~~"~~top~~"	+	# "K562 1": 1:4 dilution cells, no DNA
-	~~\| <u>Reagent</u> \|\| <u>Volume</u>~~	+	# "K562 1": 1:8 dilution cells, +DNA
-	\|-	+	# "K562 1": 1:8 dilution cells, no DNA
-	~~\| reagent~~ 1 ~~\|\| # μL~~	+
-	\|-	+
-	~~\| reagent 2 \|\| #~~	+
-	\|-	+
-	~~\| reagent 3 \|\| #~~	+
-	\|-	+
-	~~\| reagent~~ 4 ~~\|\| #~~	+
-	\|-	+
-	~~\| dH<sub>2</sub>O \|\|~~ #	+
-	\|-	+
-	~~\|   \|\|~~ # μL	+
-	\|}	+
-		+
-	~~--> Reaction conditions~~	+

		+	* Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
		+	* Pellet cells at r.t./ 3 min./ 2000 rpm
		+	* Discard supernatant
		+	* Resuspend pellet in 500 μL TriZOL
		+	* Store at -80°C for next steps (Carly)

Karmella Haynes: Autocreate 2012/12/18 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics

Karmella Haynes — Tue, 18 Dec 2012 22:00:46 GMT

Autocreate 2012/12/18 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]Pc-TF Genomics
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]] {{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|


==mm/dd/yy==

* Line item 1

----
'''Line item 1''' 
> Samples

{| class="wikitable" border="0" cellspacing="3" 
|-valign="top"
| Reagent || Volume
|-
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH2O || #
|-
|   || # μL
|}

--> Reaction conditions


|}

__NOTOC__