The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Pc-TF Genomics
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
12/18/12
- TRIzol-RNeasy prep: lyse cells in TriZOL; store at -80°C
TRIzol-RNeasy prep
- "K562 1": 1:2 dilution cells, +DNA
- "K562 1": 1:2 dilution cells, no DNA
- "K562 1": 1:4 dilution cells, +DNA
- "K562 1": 1:4 dilution cells, no DNA
- "K562 1": 1:8 dilution cells, +DNA
- "K562 1": 1:8 dilution cells, no DNA
- Collect 1 mL cells from 10 cm plate into 2.0 mL microfuge tubes (4x); discard remainder of culture
- Pellet cells at r.t./ 5 min./ 3000 rpm
- Discard supernatant
- Resuspend pellet in 500 μL TRIzol
- Store at -80°C for next steps (Carly)
|