User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/27

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(Autocreate 2012/12/27 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
Current revision (20:05, 27 December 2012) (view source)
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==mm/dd/yy==
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==12/27/12==
<!-- Precede finished items with a checkmark &#x2713; -->
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* Line item 1
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* PcTF transfection: SK-N-SH TRIzol prep
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'''Line item 1'''<br>
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'''SK-N-SH TRIzol prep'''<br>
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> Samples
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# SK-N-SH, 1, +DNA
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# SK-N-SH, 2, (-)
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
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|-valign="top"
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| <u>Reagent</u> || <u>Volume</u>
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|-
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| reagent 1 || # μL
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| reagent 2 || #
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|-
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| reagent 3 || #
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|-
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| reagent 4 || #
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|-
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| dH<sub>2</sub>O || #
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| &nbsp; || # μL
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|}
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--> Reaction conditions
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* Discarded growth medium
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* Added 500 μL TRIzol directly to wells
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* incubated at r.t. for ~5 min.
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* Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
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* Transferred to 2.0 mL tubes. Stored at -80°C

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12/27/12

  • PcTF transfection: SK-N-SH TRIzol prep

SK-N-SH TRIzol prep

  1. SK-N-SH, 1, +DNA
  2. SK-N-SH, 2, (-)
  • Discarded growth medium
  • Added 500 μL TRIzol directly to wells
  • incubated at r.t. for ~5 min.
  • Collected lysed cells from wells with gentle scraping (using 1000 μL pipette)
  • Transferred to 2.0 mL tubes. Stored at -80°C



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