User:Karmella Haynes/Notebook/PcTF Genomics/2012/12/27: Difference between revisions
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* | * PcTF transfection: SK-N-SH TRIzol prep | ||
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''' | '''SK-N-SH TRIzol prep'''<br> | ||
# SK-N-SH, 1, +DNA | |||
# SK-N-SH, 2, (-) | |||
* Discarded growth medium | |||
* Added 500 μL TRIzol directly to wells | |||
* incubated at r.t. for ~5 min. | |||
* Collected lysed cells from wells with gentle scraping (using 1000 μL pipette) | |||
* Transferred to 2.0 mL tubes. Stored at -80°C | |||
Revision as of 17:05, 27 December 2012
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12/27/12
SK-N-SH TRIzol prep
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