User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/05

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From the Haynes & Silver JBC 2011 paper, ChIP-seq experiment<br><br>
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Based on Carly's control gene list [http://openwetware.org/wiki/Haynes_Lab:Notebook/Synthetic_Biology_and_Bioinformatics_for_Predictable_Control_of_Therapeutic_Genes/2012/05/28]; CDKN2A and MMP12 added by me<br><br>
{| {{table}}
{| {{table}}
|-
|-
| Gene || Chromosome || Expected state || Observed max. DOC*
| Gene || Chromosome || Expected state || Observed max. DOC*
 +
|-
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| ALAS1 || chr 3 || active || ---
 +
|-
 +
| TBP || chr 6 || active || ---
 +
|-
 +
| ACTB || chr 7 || active || ---
 +
|-
 +
| GUSB || chr 7 || active || ---
 +
|-
 +
| PPIA || chr 7 || active || ---
 +
|-
 +
| GAPDH || chr 12 || active || ---
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|-
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| B2M || chr 15 || active || ---
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|-
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| G6PD || chr 15 || active || ---
 +
|-
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| HPRT1 || chr X (24) || active || ---
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|-
 +
| PGK1 ||  chr X (24) || active || ---
|-
|-
| CDKN2A || chr9 || silent (H3K27me3+) || ---
| CDKN2A || chr9 || silent (H3K27me3+) || ---
|-
|-
| MMP12 || chr11 || silent (H3K27me3+) || ---
| MMP12 || chr11 || silent (H3K27me3+) || ---
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|-
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| MYT1 || chr20 || silent (H3K27me3+) || ---
 +
|-
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| OLIG1 || chr21 || silent (H3K27me3+) || ---
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|-
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| OLIG2 || chr21 || silent (H3K27me3+) || ---
|}
|}

Revision as of 21:25, 5 January 2013

Pc-TF Genomics Main project page
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01/05/13

  • ChIP seq analysis: bird's eye view of H3K27me3 enrichment
  • ChIP-seq enrichment: PcTF vs. PcTF mock for select genes

H3K27me3 enrichment

  • Completed H3K27me3 (lane 3) "BAM --> assembly" workflow
  • Open in SeqMan pro to explore known silenced vs. active genes


Workflow

  • Open SeqMan Pro. File > Open > ...navigate to the desired ###.assembly file in the SeqMan NGen Projects folder. Processing takes 5 -10 minutes.
  • In an internet browser, go to the UCSC genome browser. Select Feb. 2009 GRCh37/hg19.
  • Enter a gene name. Click [Submit].
  • Take note of the chromosome number.
  • Find the appropriate assembly template in the SeqMan Pro (e.g., NC_000001 for chromosome 1). Double click it.
  • Select Contig > Strategy View to view the enrichment histogram.
  • Select Features > Show Feature Table. Find the gene name. Click on it and the strategy view window will jump to that gene.
  • Click the gene symbol. Zoom in. Tak emote of the maximum depth of coverage (max. DOC).


Based on Carly's control gene list [1]; CDKN2A and MMP12 added by me

Gene Chromosome Expected state Observed max. DOC*
ALAS1 chr 3 active ---
TBP chr 6 active ---
ACTB chr 7 active ---
GUSB chr 7 active ---
PPIA chr 7 active ---
GAPDH chr 12 active ---
B2M chr 15 active ---
G6PD chr 15 active ---
HPRT1 chr X (24) active ---
PGK1 chr X (24) active ---
CDKN2A chr9 silent (H3K27me3+) ---
MMP12 chr11 silent (H3K27me3+) ---
MYT1 chr20 silent (H3K27me3+) ---
OLIG1 chr21 silent (H3K27me3+) ---
OLIG2 chr21 silent (H3K27me3+) ---




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