User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/07: Difference between revisions
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# Open '''Array Star'''. ''Windows only. This can be run on Parallels from a Mac.'' | # Open '''Array Star'''. ''Windows only. This can be run on Parallels from a Mac.'' | ||
# Click "Start Chip-Seq project..." | # Click "Start Chip-Seq project..." | ||
# Click [Add File..] | # Add Experiments to Import: Click [Add File..] | ||
# Select a ###.bam file from the ChiP seq data folder (shared desktop). Click [Next >]. | # Select a ###.bam file from the ChiP seq data folder (shared desktop). Click [Next >]. | ||
# Create binding proteins: Click [Create Binding Protein]. Binding protein label = the name of the protein that was pulled down by the antibody via ChIP. Binding site type = Unknown. | # Create binding proteins: Click [Create Binding Protein]. Binding protein label = the name of the protein that was pulled down by the antibody via ChIP. Binding site type = Unknown. Click [OK]. | ||
Click [ | # Assign Binding Proteins to Experiments: Protein label = the appropriate binding protein (created in previous step). Click [Next >]. | ||
'''PcTF vs. H3K27me3''' | |||
# Add Experiments to Import: added 6_aln_sorted.bam (H3K27me3) and 1_aln_sorted.bam (PcTF) | |||
# Create binding proteins: created "PcTF" for 1_aln_sorted, and "H3K27me3" for 6_aln_sorted. | |||
Revision as of 19:32, 7 January 2013
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01/07/13
Array Star analysis
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