User:Karmella Haynes/Notebook/PcTF Genomics/2013/01/07

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


01/07/13

  • Array Star analysis

Array Star analysis

  1. Open Array Star. Windows only. This can be run on Parallels from a Mac.
  2. Click "Start Chip-Seq project..."
  3. Add Experiments to Import: Click [Add File..]
  4. Select a ###.bam file from the ChiP seq data folder (shared desktop). Click [Next >].
  5. Create binding proteins: Click [Create Binding Protein]. Binding protein label = the name of the protein that was pulled down by the antibody via ChIP. Binding site type = Unknown. Click [OK].
  6. Assign Binding Proteins to Experiments: Protein label = the appropriate binding protein (created in previous step). Click [Next >].


PcTF vs. H3K27me3

  1. Add Experiments to Import: added 6_aln_sorted.bam (H3K27me3) and 1_aln_sorted.bam (PcTF)
  2. Create binding proteins: created "PcTF" for 1_aln_sorted, and "H3K27me3" for 6_aln_sorted.