User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/02: Difference between revisions
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---- | ---- | ||
'''Gal4EED/luc test'''<br> | '''Gal4EED/luc test'''<br> | ||
> Dox dose response test | > Dox dose response test<br> | ||
> 6-well plate (plated 5/29/13), shipment 1 cells | |||
{| class="wikitable" border="0" cellspacing="5" <!-- Lipofectamine transfection table --> | {| class="wikitable" border="0" cellspacing="5" <!-- Lipofectamine transfection table --> | ||
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| <u>Wells</u> || <u>dox μg/mL</u> || <u>Volume dox (1 mg/mL) in 3.5 mL medium</u> | | <u>Wells</u> || <u>dox μg/mL</u> || <u>Volume dox (1 mg/mL) in 3.5 mL medium</u> | ||
|- | |- | ||
| 1 || 0 | | 1 || 0 || --- | ||
|- | |- | ||
| 2 || 0.1 | | 2 || 0.1 || 0.4 μL | ||
|- | |- | ||
| 3 || 0.2 | | 3 || 0.2 || 0.8 μL | ||
|- | |- | ||
| 4 || 0.5 | | 4 || 0.5 || 1.8 μL | ||
|- | |- | ||
| 5 || 1.0 | | 5 || 1.0 || 3.5 μL | ||
|- | |- | ||
| 6 || 2.0 | | 6 || 2.0 || 7.0 μL | ||
|} | |} | ||
Revision as of 00:46, 3 June 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/02/13
Lipo transfection
> Add 570 μL Opti-MEM to each 20 μL DNA sample
Gal4EED/luc test
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