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 Pc-TF Genomics
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06/10/13
- Split cells: Gal4-EED/luc shipment 1 and shipment 2 T-75 flasks (1:5)
- Split dox induced cells
Gal4EED/luc test
> Dox dose response test
6-well plates for luc activity assays & flow cytometry, etc.
- 6-well plate (originally plated 5/29/13), shipment 1 cells
- Split 1:4 into 2 new 6-well plates: resuspend detached cells in a final volume of 4 mL puro+ medium, add 1 mL cells to 3mL fresh medium +proper amount of dox
| Wells |
dox μg/mL |
Volume dox (1 mg/mL) in 4.0 mL medium
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| 1 |
0 |
---
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| 2 |
0.1 |
0.5 μL
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| 3 |
0.2 |
1.0 μL
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| 4 |
0.5 |
2.0 μL
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| 5 |
1.0 |
4.0 μL
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| 6 |
2.0 |
8.0 μL
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24-well plate for microscopy:
- Use leftover suspension from 6-well plating
- For each sample (1-6) add 0.5 mL suspension to 0.5 mL fresh medium +proper amount of doc in 4 wells
- Cells will be fixed and stained directly in the plate, following David Dreher's protocol.
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Pc-TF Genomics
