User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/12: Difference between revisions
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> Add 7.5 μL Lipofectamine LTX to each DNA+Opti-MEM+PLUS reagent sample. 30 min/ room temp <br> | > Add 7.5 μL Lipofectamine LTX to each DNA+Opti-MEM+PLUS reagent sample. 30 min/ room temp <br> | ||
> Add 600 μL complexes to each well (final volume of medium = 4 ml/well)<br> | > Add 600 μL complexes to each well (final volume of medium = 4 ml/well)<br> | ||
> Grow cells at 37°C overnight<br> | > Grow cells at 37°C overnight<br> | ||
Revision as of 14:02, 30 June 2013
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06/12/13
Gal4-EED/luc cells IFC
Lipo transfection
> Add 570 μL Opti-MEM to each 20 μL DNA sample
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