User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/15: Difference between revisions
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* Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining | * Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining | ||
* Gal4-EED/ luc dox induction - shipment 2 cells | * Gal4-EED/ luc dox induction - shipment 2 cells | ||
* PcTF transfection for U2OS, outgrowth #1 | |||
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# 1.0 | # 1.0 | ||
# 2.0 (induced on 6/02/13, 13 days) | # 2.0 (induced on 6/02/13, 13 days) | ||
* Followed standard protocol | * Followed standard protocol | ||
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* For this set, test earlier time points and try luc activity assay | * For this set, test earlier time points and try luc activity assay | ||
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'''PcTF transfection for U2OS, outgrowth #1''' | |||
* 6-well plates: 1-3 = +PcTF, 4-6 = mock (lipo only) | |||
* Expand cells from 6-well plates in individual 10 cm plates (10 mL medium). 6 10 cm plates total. | |||
* Use standard growth medium. | |||
* Use all cells. Do not dilute. | |||
Revision as of 20:11, 26 June 2013
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06/15/13
Gal4-EED/luc Luciferase ICC Samples (Shipment 1 cells)
Gal4-EED/luc dox induction Samples (Shipment 2 cells); new 6-well plate...
PcTF transfection for U2OS, outgrowth #1
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