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Pc-TF Genomics
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06/15/13
- Gal4-EED/luc Luciferase ICC - fixation, permeabilization, primary ab staining
- Gal4-EED/ luc dox induction - shipment 2 cells
Gal4-EED/luc Luciferase ICC
Samples (Shipment 1 cells)
- 0 μg/mL dox
- 0.1
- 0.2
- 0.5
- 1.0
- 2.0 (induced on 6/02/13, 13 days)
- Harvested cells from one 6-well plate (split plate #2 for further analyses)
- At the primary antibody staining step, keep samples and reagents on ice overnight in the cold room
Gal4-EED/luc dox induction
Samples (Shipment 2 cells); new 6-well plate...
- 0 μg/mL dox
- 0.1
- 0.2
- 0.5
- 1.0
- 2.0 (induced on 6/02/13, 13 days)
- For this set, test earlier time points and try luc activity assay
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