User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/18: Difference between revisions
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* QUICKLY Added 100 μL of complete steady-luc buffer to each sample (pipetted up/down once to mix) | * QUICKLY Added 100 μL of complete steady-luc buffer to each sample (pipetted up/down once to mix) | ||
* Incubated at room temp/ 10 min. | * Incubated at room temp/ 10 min. | ||
* Created new protocol: KAH_luciferase | * Synergy H1 reader - Created new protocol: KAH_luciferase | ||
** Optimized specs: | ** Optimized specs: Luminescence, Endpoint, Full plate, Integration time 0:01.00 (MM:SS.ss), Filter set 1, Emission: full light, Optics: top, '''Gain 200''', Read Speed: normal, Delay: 100 msec, Extended Dynamic Range, Read Height: 1 mm | ||
Revision as of 19:42, 18 June 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
06/18/13
Luciferase activity assay - time point 1
--> Reaction conditions
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