User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/18: Difference between revisions
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<u>Luc assay</u> | <u>Luc assay</u> | ||
* Filtered 500 μL cells through strainer caps (<font color="red">should use | * Filtered 500 μL cells through strainer caps (<font color="red">should use 600 μL next time</font>) | ||
* Transferred 100 μL strained cells to 3 wells in 96-well plate (black walls; clear, flat bottom) | * Transferred 100 μL strained cells to 3 wells in 96-well plate (black walls; clear, flat bottom) | ||
** Saved remainder of strained cells for flow cytometry/ counting | ** Saved remainder of strained cells for flow cytometry/ counting | ||
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| <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | | <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | ||
|- | |- | ||
| sample 1 || 8077 || x5 = || | | sample 1 || 8077 || x5 = || 40,385 | ||
|- | |- | ||
| sample 2 || | | sample 2 || 9552 || x5 = || 47,760 | ||
|- | |- | ||
| sample 3 || | | sample 3 || 9460 || x5 = || 47,300 | ||
|- | |- | ||
| sample 4 || | | sample 4 || 3608 || x5 = || 18,040 | ||
|- | |- | ||
| sample 5 || | | sample 5 || 4723 || x5 = || 23,615 | ||
|- | |- | ||
| sample 6 || | | sample 6 || 5851 || x5 = || 29,255 | ||
|} | |} | ||
Revision as of 19:44, 18 June 2013
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06/18/13
Luciferase activity assay - time point 1
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