User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/19

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(mm/dd/yy)
(06/19/13)
Line 41: Line 41:
| <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || &nbsp; || <u>Cells/ 100 μL</u>
| <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || &nbsp; || <u>Cells/ 100 μL</u>
|-
|-
-
| sample 1 || || x5 = ||  
+
| sample 1 || 10,880 || x5 = ||  
|-
|-
-
| sample 2 || || x5 = ||  
+
| sample 2 || 10,430 || x5 = ||  
|-
|-
-
| sample 3 || || x5 = ||  
+
| sample 3 || 11,897 || x5 = ||  
|-
|-
-
| sample 4 || || x5 = ||  
+
| sample 4 || 10,316 || x5 = ||  
|-
|-
| sample 5 || || x5 = ||  
| sample 5 || || x5 = ||  

Revision as of 00:38, 20 June 2013

Pc-TF Genomics Main project page
Previous entry      Next entry


06/19/13

  • Luc assay, time point 2

Luciferase activity assay - time point 2


Cell prep

  • Harvested cells
    • Seeded new dox+ plate with 1/2 of cells
    • Pelleted other half, resuspended in 2 mL FACS buffer


Assay reagents

  • Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as 6/18/13)


Luc assay

  • Filtered 700 μL cells through strainer caps
  • Other steps same as 6/18/13


Cell counts

  • Wang lab's Accuri flow cytometer.
  • Set machine to read 20 uL of cells
  • Be sure to "clean" with water-run in between samples


Sample ID Gated count/ 20 μL   Cells/ 100 μL
sample 1 10,880 x5 =
sample 2 10,430 x5 =
sample 3 11,897 x5 =
sample 4 10,316 x5 =
sample 5 x5 =
sample 6 x5 =



Personal tools