User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/19: Difference between revisions
From OpenWetWare
(6 intermediate revisions by the same user not shown) | |||
Line 11: | Line 11: | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* Luc assay, time point 2 | * Luc assay, time point 2 | ||
* PcTF transfection for U2OS, outgrowth #2 | |||
---- | ---- | ||
Line 41: | Line 43: | ||
| <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | | <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | ||
|- | |- | ||
| sample 1 || || x5 = || | | sample 1 || 10,880 || x5 = || 54,400 | ||
|- | |- | ||
| sample 2 || || x5 = || | | sample 2 || 10,430 || x5 = || 52,150 | ||
|- | |- | ||
| sample 3 || || x5 = || | | sample 3 || 11,897 || x5 = || 59,485 | ||
|- | |- | ||
| sample 4 || || x5 = || | | sample 4 || 10,316 || x5 = || 51,580 | ||
|- | |- | ||
| sample 5 || || x5 = || | | sample 5 || 9,784 || x5 = || 48,920 | ||
|- | |- | ||
| sample 6 || || x5 = || | | sample 6 || 11,549 || x5 = || 57,745 | ||
|} | |} | ||
---- | |||
'''PcTF transfection for U2OS, outgrowth #2''' | |||
* Expanded transfected and mock-transfected cells from old 10 cm plates (6/15/13) | |||
* Used only samples 1 (+PcTF) and 4 (mock) | |||
* 1:10 passage into three new 10 cm plates labeled "1-A, 1-B, 1-C" (+PcTF) and "4-A, 4-B, 4-C" (mock) | |||
Revision as of 20:13, 26 June 2013
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||
06/19/13
Luciferase activity assay - time point 2
PcTF transfection for U2OS, outgrowth #2
|