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 Pc-TF Genomics
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06/19/13
Luciferase activity assay - time point 2
Cell prep
- Harvested cells
- Seeded new dox+ plate with 1/2 of cells
- Pelleted other half, resuspended in 2 mL FACS buffer
Assay reagents
- Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as 6/18/13)
Luc assay
- Filtered 700 μL cells through strainer caps
- Other steps same as 6/18/13
Cell counts
- Wang lab's Accuri flow cytometer.
- Set machine to read 20 uL of cells
- Be sure to "clean" with water-run in between samples
| Sample ID |
Gated count/ 20 μL |
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Cells/ 100 μL
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| sample 1 |
10,880 |
x5 = |
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| sample 2 |
10,430 |
x5 = |
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| sample 3 |
11,897 |
x5 = |
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| sample 4 |
10,316 |
x5 = |
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| sample 5 |
9,784 |
x5 = |
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| sample 6 |
11,549 |
x5 = |
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Pc-TF Genomics
