User:Karmella Haynes/Notebook/PcTF Genomics/2013/06/30: Difference between revisions
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> Cells were 100% confluent<br> | > Cells were 100% confluent<br> | ||
> Re-plate 1:2 dilution prior to transfection in 3.4 mL p/s-free medium<br> | > Re-plate 1:2 dilution prior to transfection in 3.4 mL p/s-free medium<br> | ||
> Transfect human PcTF under control of constitutive CMV-TetO promoter (KAH126/MV2); | > Transfect human PcTF under control of constitutive CMV-TetO promoter (KAH126/MV2); 226 ng/μL<br>; Note: no TetR in SK-N-SH, so expression will be constitutive | ||
Revision as of 14:02, 30 June 2013
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6/30/13/dd/yy
Gal4-EED/luc cells IFC
Lipo transfection
> Add 570 μL Opti-MEM to each 20 μL DNA sample
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