User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/04: Difference between revisions
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<u>Assay reagents</u> | <u>Assay reagents</u> | ||
* Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/ | * Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/06/18 6/18/13]) | ||
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* Samples loaded in triplicate (by columns) | * Samples loaded in triplicate (by columns) | ||
* Included luc buffer + FACS-buffer "blank" sample (well D1) | * Included luc buffer + FACS-buffer "blank" sample (well D1) | ||
* Other steps same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/ | * Other steps same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/06/18 6/18/13] | ||
Revision as of 20:08, 6 July 2013
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07/04/13
Luciferase activity assay - time point: 4 days
Gal4-EED/luc Dox induction #2
Addition of serial dilutions of dox to cells
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