User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/06: Difference between revisions
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* Gal4-EED/luc dox time point (48 hours) | * Gal4-EED/luc dox time point (48 hours) | ||
* Gal4-EED/luc PcTF transfection - microscopy & flow cytometry | * Gal4-EED/luc PcTF transfection - microscopy & flow cytometry | ||
* SK-N-SH +PcTF - TRIzol prep #1 | |||
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<u>Assay reagents</u> | <u>Assay reagents</u> | ||
* Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/ | * Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/06/18 6/18/13]) | ||
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* Samples loaded in triplicate (by columns) | * Samples loaded in triplicate (by columns) | ||
* Included luc buffer + FACS-buffer "blank" sample (well D1) | * Included luc buffer + FACS-buffer "blank" sample (well D1) | ||
* Other steps same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/ | * Other steps same as [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2013/06/18 6/18/13] | ||
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| <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | | <u>Sample ID</u> || <u>Gated count/ 20 μL</u> || || <u>Cells/ 100 μL</u> | ||
|- | |- | ||
| sample 1 || | | sample 1 || 37,629 || x5 = || | ||
|- | |- | ||
| sample 2 || | | sample 2 || 34,580 || x5 = || ### | ||
|- | |- | ||
| sample 3 || | | sample 3 || 40,096 || x5 = || | ||
|- | |- | ||
| sample 4 || | | sample 4 || 46,599 || x5 = || | ||
|- | |- | ||
| sample 5 || | | sample 5 || 46,491 || x5 = || | ||
|- | |- | ||
| sample 6 || | | sample 6 || 46,254 || x5 = || | ||
|} | |} | ||
Revision as of 20:48, 6 July 2013
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07/06/13
Luciferase activity assay - time point: 2 days Cell prep
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