The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Pc-TF Genomics
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
07/06/13
- Gal4-EED/luc dox time point (48 hours)
- Gal4-EED/luc PcTF transfection - microscopy & flow cytometry
Luciferase activity assay - time point: 2 days
Cell prep
- Harvested cells (induced on 7/04/13)
- Seeded new 2 new plates with non-induced cells from 7/04/13
- Pelleted all of the dox+ cells, resuspended in 2 mL FACS buffer
Assay reagents
- Used the Biotium Steady-Luc Firefly HTS Assay Kit (same as 6/18/13)
Luc assay
- Filtered 700 μL cells through strainer caps
- Used opaque white Costar plate (from Rege lab)
- Samples loaded in triplicate (by columns)
- Included luc buffer + FACS-buffer "blank" sample (well D1)
- Other steps same as 6/18/13
Cell counts
- Wang lab's Accuri flow cytometer.
- Set machine to read 20 uL of cells
- Be sure to "clean" with water-run in between samples
Sample ID |
Gated count/ 20 μL |
|
Cells/ 100 μL
|
sample 1 |
37,629 |
x5 = |
|
sample 2 |
34,580 |
x5 = |
###
|
sample 3 |
|
x5 = |
|
sample 4 |
|
x5 = |
|
sample 5 |
|
x5 = |
|
sample 6 |
|
x5 = |
|
|