User:Karmella Haynes/Notebook/PcTF Genomics/2013/07/06: Difference between revisions
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* Gal4-EED/luc dox time point (48 hours) | * Gal4-EED/luc dox time point (48 hours) | ||
* Gal4-EED/luc PcTF transfection - microscopy & flow cytometry | * Gal4-EED/luc PcTF transfection - microscopy & flow cytometry | ||
* SK-N-SH +PcTF - TRIzol prep #1 | * SK-N-SH +PcTF & mock - TRIzol prep #1 | ||
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| sample 6 || 46,254 || x5 = || | | sample 6 || 46,254 || x5 = || | ||
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'''SK-N-SH TRIzol prep'''<br> | |||
# +PcTF, plate 1 | |||
# mock, plate 4 | |||
(Cells may need longer to express genes. Previous expt. was done after ~10 days. Will do this prep just in case cell culture declines) | |||
* Used other two plates to passage cells ~1:5 (will prep after returning from SB6.0) | |||
* Cells were 100% confluent | |||
* Discarded growth medium | |||
* Added 2 mL TRIzol directly to plates | |||
* Incubated at r.t. for ~5 min. | |||
* Collected lysed cells from plate with gentle scraping (pipette tip) | |||
* Transferred 500 μL aliquots to 2.0 mL tubes. Stored at -80°C | |||
** 4x PcTF+ samples | |||
** 4x mock samples | |||
Revision as of 21:06, 6 July 2013
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07/06/13
Luciferase activity assay - time point: 2 days Cell prep
SK-N-SH TRIzol prep
(Cells may need longer to express genes. Previous expt. was done after ~10 days. Will do this prep just in case cell culture declines)
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