User:Karmella Haynes/Notebook/PcTF Genomics/2014/01/27: Difference between revisions

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{|
{|
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|  
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Gene Target'''
| align="center" style="background:#f0f0f0;"|'''Gene Target'''
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|-
|-
| Rxn 24:||no template||ref. gene, GAPD
| Rxn 24:||no template||ref. gene, GAPD
|}
'''Primer/Probe Master Mixes (8 total)'''
{|
| align="center" style="background:#f0f0f0;"|'''Reagent'''
| align="center" style="background:#f0f0f0;"|'''(Single well)'''
| align="center" style="background:#f0f0f0;"|'''Gene Target 1 - 7 (x10)'''
| align="center" style="background:#f0f0f0;"|'''Gene Target GAPD (x10)'''
|-
| 2x LC480 Probes Master||(7.5 μL)||75||75
|-
| 20 μM Forward primer||(0.3 μL)||3||3.0 GAPD primers*
|-
| 20 μM Reverse primer||(0.3 μL)||3||---
|-
| 10 μM UPL probe||(0.3 μL)||3||3.0 GAPD UPL probe*
|-
| PCR H2O||(0.1 μL)||1||4
|-
| Total vol.||(8.5 μL)||85||85
|}
|}


Revision as of 16:52, 24 January 2014

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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01/27/14

  • qRT-PCR experiment - PlateK562_1/ U2OS

qRT-PCR - Plate K562_1/ U2OS


  Template Gene Target
Rxn 1: treated cells KIT, primers 13/14
Rxn 2: treated cells TNFRSF11A, primers 15/16
Rxn 3: treated cells EGFR, primers 17/18
Rxn 4: treated cells WT1, primers 19/20
Rxn 5: treated cells HLF, primer 21/22
Rxn 6: treated cells BCL6, primer 23/24
Rxn 7: treated cells mCh
Rxn 8: treated cells ref. gene, GAPD
Rxn 9: untreated cells KIT, primers 13/14
Rxn 10: untreated cells TNFRSF11A, primers 15/16
Rxn 11: untreated cells EGFR, primers 17/18
Rxn 12: untreated cells WT1, primers 19/20
Rxn 13: untreated cells HLF, primer 21/22
Rxn 14: untreated cells BCL6, primer 23/24
Rxn 15: untreated cells mCh
Rxn 16: untreated cells ref. gene, GAPD
Rxn 17: no template KIT, primers 13/14
Rxn 18: no template TNFRSF11A, primers 15/16
Rxn 19: no template EGFR, primers 17/18
Rxn 20: no template WT1, primers 19/20
Rxn 21: no template HLF, primer 21/22
Rxn 22: no template BCL6, primer 23/24
Rxn 23: no template mCh
Rxn 24: no template ref. gene, GAPD


Primer/Probe Master Mixes (8 total)

Reagent (Single well) Gene Target 1 - 7 (x10) Gene Target GAPD (x10)
2x LC480 Probes Master (7.5 μL) 75 75
20 μM Forward primer (0.3 μL) 3 3.0 GAPD primers*
20 μM Reverse primer (0.3 μL) 3 ---
10 μM UPL probe (0.3 μL) 3 3.0 GAPD UPL probe*
PCR H2O (0.1 μL) 1 4
Total vol. (8.5 μL) 85 85



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