User:Karmella Haynes/Notebook/PcTF Genomics/2014/01/27: Difference between revisions
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* qRT-PCR experiment - PlateK562_1/ U2OS | * qRT-PCR experiment - PlateK562_1/ U2OS | ||
* U2OS RT-PCR - RNA extraction & cDNA synthesis | |||
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'''RNA Extraction''' | |||
* Use RNeasy Mini Kit | |||
Samples (TriZOL-lysed U2OS samples from -80°C freezer) | |||
# Tube 1-A (+PcTF) | |||
# Tube 1-A (+PcTF) | |||
# Tube 4-A (mock) | |||
# Tube 4-A (mock) | |||
Procedure notes | |||
* | |||
'''cDNA Synthesis''' | |||
* Use Superscript III kit (freezer) | |||
Procedure notes | |||
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Revision as of 17:25, 3 February 2014
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01/27/14
RNA Extraction
Samples (TriZOL-lysed U2OS samples from -80°C freezer)
Procedure notes
cDNA Synthesis
Procedure notes qRT-PCR - Plate K562_1/ U2OS
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