User:Karmella Haynes/Notebook/PcTF Genomics/2014/02/12: Difference between revisions

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* Plasmid transformations
* Plasmid transformations
* Benchling entry




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'''Plasmid Transformations'''<br>
'''Plasmid Transformations'''<br>
# CMV/V0120 - Benchling link: https://benchling.com/hayneslab/mammalian-basic-parts/cmv/
# CMV/V0120 - Benchling link: https://benchling.com/hayneslab/mammalian-basic-parts/cmv/
# MV9 vector - need to create Benchling link
# MV9 vector - BioBrick [http://parts.igem.org/Part:BBa_J176171 BBa_J176171]
# water negative control
# water negative control


Revision as of 12:22, 12 February 2014

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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02/12/14

  • Plasmid transformations


Plasmid Transformations

  1. CMV/V0120 - Benchling link: https://benchling.com/hayneslab/mammalian-basic-parts/cmv/
  2. MV9 vector - BioBrick BBa_J176171
  3. water negative control


Reagent 1 2 3
DNA 0.5 0.5 ---
dH2O 10.0 10.0 10.0
  10.5 10.5 10.0
  • Add 50 μL DH5α-turbo cells (thawed on ice)
  • Incubate on ice for 5 min.
  • Plate on 100 μg/mL amp LB agar



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