User:Karmella Haynes/Notebook/PcTF Genomics/2014/02/12

From OpenWetWare

< User:Karmella Haynes | Notebook | PcTF Genomics | 2014 | 02(Difference between revisions)
Jump to: navigation, search
(02/12/14)
Current revision (19:09, 13 February 2014) (view source)
(02/12/14)
 
Line 39: Line 39:
* Plates looked great. Put back into 37°C incubator for two more hours.
* Plates looked great. Put back into 37°C incubator for two more hours.
-
[[Image:KAH021314_plates.jpg|300px]]
+
[[Image:KAH021314_plates.jpg|400px]]

Current revision

Pc-TF Genomics Main project page
Previous entry      Next entry


02/12/14

  • Plasmid transformations



Plasmid Transformations

  1. CMV/V0120 - BioBrick CMV, Benchling part: CMV
  2. MV9 vector - BioBrick BBa_J176171, Benchling part: MV9
  3. water negative control


Reagent 1 2 3
DNA 0.5 0.5 ---
dH2O 10.0 10.0 10.0
  10.5 10.5 10.0
  • Add 50 μL DH5α-turbo cells (thawed on ice)
  • Incubate on ice for 5 min.
  • Plate on 100 μg/mL amp LB agar
  • Took plates out of 37°C after ~4 hours and placed on bench at room temp to prevent over-growing

2/13/14

  • Plates looked great. Put back into 37°C incubator for two more hours.



Personal tools