User:Karmella Haynes/Notebook/PcTF Genomics/2014/02/14: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/02/14 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
Line 8: Line 8:




==mm/dd/yy==
==02/14/14==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* Miniprep digests: CMV/V0120, MV9


----
----
'''Line item 1'''<br>
'''Miniprep Digests'''<br>
> Samples
# CMV culture 1 - check with E/P
# CMV culture 2 - check with E/P
# MV9 culture 1 - check with P/X
# MV9 culture 2 - check with P/X
 
* Used the Zymo miniprep kit
* Pelleted each 5 mL culture at 2000 rpm/ 5 min in old centrifuge
* Resuspended each pellet in 600 μL dH<sub>2</sub>O before miniprep


{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->

Revision as of 15:35, 14 February 2014

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


02/14/14

  • Miniprep digests: CMV/V0120, MV9

Miniprep Digests

  1. CMV culture 1 - check with E/P
  2. CMV culture 2 - check with E/P
  3. MV9 culture 1 - check with P/X
  4. MV9 culture 2 - check with P/X
  • Used the Zymo miniprep kit
  • Pelleted each 5 mL culture at 2000 rpm/ 5 min in old centrifuge
  • Resuspended each pellet in 600 μL dH2O before miniprep
Reagent Volume
reagent 1 # μL
reagent 2 #
reagent 3 #
reagent 4 #
dH2O #
  # μL

--> Reaction conditions



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karmella_Haynes/Notebook/PcTF_Genomics/2014/02/14&oldid=772579"