User:Karmella Haynes/Notebook/PcTF Genomics/2014/04/02: Difference between revisions

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* Refer to [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2014/02/10 02/10/14] for the procedure and Command parameters
* Refer to [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2014/02/10 02/10/14] for the procedure and Command parameters
* Refer to [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2011/12/19 12/19/11] for Hi-Seq parameters: fragment length (bioanalyzer result), read length
* Refer to [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/PcTF_Genomics/2011/12/19 12/19/11] for Hi-Seq parameters: fragment length (bioanalyzer result), read length
* Input data must be
* Example '''Command''' syntax: java -jar STAR.jar 100 1 true .5 10 true test_data1.txt false false test_out 1 true 10 5 0


* Command: java -jar STAR.jar 100 1 true .5 10 true test_data1.txt false false test_out 1 true 10 5 0
{| {{table}}
{| {{table}}
|-
|-
| Parameter || Trail 1
| Parameter || Command 1
|-
|-
| Extension length || fragment length 200 - read length 100 = '''100'''
| Extension length || fragment length 200 - read length 100 = '''100'''
** Start coordinate = '''1'''
|-
| Start coordinate || 1
** Right end inclusion = '''true'''
** Right end inclusion = '''true'''
** FDR cut-off = '''.5'''
** FDR cut-off = '''.5'''

Revision as of 13:41, 2 April 2014

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04/02/14

  • STAR analysis of my ChIP-seq data



STAR analysis

  • Refer to 02/10/14 for the procedure and Command parameters
  • Refer to 12/19/11 for Hi-Seq parameters: fragment length (bioanalyzer result), read length
  • Input data must be
  • Example Command syntax: java -jar STAR.jar 100 1 true .5 10 true test_data1.txt false false test_out 1 true 10 5 0
Parameter Command 1
Extension length fragment length 200 - read length 100 = 100
Start coordinate 1
    • Right end inclusion = true
    • FDR cut-off = .5
    • Num perms = 10