User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/14: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/05/14 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
Line 8: Line 8:




==mm/dd/yy==
==05/14/14==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* RT-PCR: QC_plate, all cDNA batches


----
----
'''Line item 1'''<br>
'''RT-PCR: QC_plate, all cDNA batches'''<br>
> Samples
* Run mCh, GAPD reactions on all cDNA batches, all cell lines: 16 total
* Will be used to compare cDNA quality, PcTF expression, and generate reference values
 
* File name:
* mCherry wells: 16x3 = 48
* GAPD wells: 16x3 = 48


{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->

Revision as of 17:37, 14 May 2014

Pc-TF Genomics <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


05/14/14

  • RT-PCR: QC_plate, all cDNA batches

RT-PCR: QC_plate, all cDNA batches

  • Run mCh, GAPD reactions on all cDNA batches, all cell lines: 16 total
  • Will be used to compare cDNA quality, PcTF expression, and generate reference values
  • File name:
  • mCherry wells: 16x3 = 48
  • GAPD wells: 16x3 = 48
Reagent Volume
reagent 1 # μL
reagent 2 #
reagent 3 #
reagent 4 #
dH2O #
  # μL

--> Reaction conditions