User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/14

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<!-- Precede finished items with a checkmark &#x2713; -->
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* RT-PCR: QC_plate, all cDNA batches
* RT-PCR: QC_plate, all cDNA batches
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* Will be used to compare cDNA quality, PcTF expression, and generate reference values
* Will be used to compare cDNA quality, PcTF expression, and generate reference values
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* File name:
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* Experiment file name:
* mCherry wells: 16x3 = 48
* mCherry wells: 16x3 = 48
* GAPD wells: 16x3 = 48
* GAPD wells: 16x3 = 48
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{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
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{|
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|-valign="top"
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| align="center" style="background:#f0f0f0;"|'''Reagent'''
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| <u>Reagent</u> || <u>Volume</u>
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| align="center" style="background:#f0f0f0;"|'''(Single well)'''
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| align="center" style="background:#f0f0f0;"|'''Gene Target mCh'''
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| align="center" style="background:#f0f0f0;"|'''Gene Target GAPD (x10)'''
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|-
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| reagent 1 || # μL
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| 2x LC480 Probes Master||(7.5 μL)||360.0||360.0
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|-
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| reagent 2 || #
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| 20 μM Forward primer||(0.3 μL)||14.4||14.4 GAPD primers
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|-
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| reagent 3 || #
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| 20 μM Reverse primer||(0.3 μL)||14.4||0
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|-
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| reagent 4 || #
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| 10 μM UPL probe||(0.3 μL)||14.4||14.4 GAPD probe
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|-
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| dH<sub>2</sub>O || #
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| PCR H2O||(0.1 μL)||4.8||19.2
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|-
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| &nbsp; || # μL
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| Total vol.||(8.5 μL)||408.0||408.0
|}
|}
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--> Reaction conditions
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Revision as of 20:38, 14 May 2014

Pc-TF Genomics Main project page
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05/14/14

  • RT-PCR: QC_plate, all cDNA batches



RT-PCR: QC_plate, all cDNA batches

  • Run mCh, GAPD reactions on all cDNA batches, all cell lines: 16 total
  • Will be used to compare cDNA quality, PcTF expression, and generate reference values
  • Experiment file name:
  • mCherry wells: 16x3 = 48
  • GAPD wells: 16x3 = 48
Reagent (Single well) Gene Target mCh Gene Target GAPD (x10)
2x LC480 Probes Master(7.5 μL)360.0360.0
20 μM Forward primer(0.3 μL)14.414.4 GAPD primers
20 μM Reverse primer(0.3 μL)14.40
10 μM UPL probe(0.3 μL)14.414.4 GAPD probe
PCR H2O(0.1 μL)4.819.2
Total vol.(8.5 μL)408.0408.0




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