User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/14: Difference between revisions
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* RT-PCR: QC_plate, all cDNA batches | * RT-PCR: QC_plate, all cDNA batches | ||
---- | ---- | ||
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* Will be used to compare cDNA quality, PcTF expression, and generate reference values | * Will be used to compare cDNA quality, PcTF expression, and generate reference values | ||
* | * Experiment file name: | ||
* mCherry wells: 16x3 = 48 | * mCherry wells: 16x3 = 48 | ||
* GAPD wells: 16x3 = 48 | * GAPD wells: 16x3 = 48 | ||
{| | {| | ||
| | | align="center" style="background:#f0f0f0;"|'''Reagent''' | ||
| | | align="center" style="background:#f0f0f0;"|'''(Single well)''' | ||
| align="center" style="background:#f0f0f0;"|'''Gene Target mCh''' | |||
| align="center" style="background:#f0f0f0;"|'''Gene Target GAPD (x10)''' | |||
|- | |- | ||
| | | 2x LC480 Probes Master||(7.5 μL)||360.0||360.0 | ||
|- | |- | ||
| | | 20 μM Forward primer||(0.3 μL)||14.4||14.4 GAPD primers | ||
|- | |- | ||
| | | 20 μM Reverse primer||(0.3 μL)||14.4||0 | ||
|- | |- | ||
| | | 10 μM UPL probe||(0.3 μL)||14.4||14.4 GAPD probe | ||
|- | |- | ||
| | | PCR H2O||(0.1 μL)||4.8||19.2 | ||
|- | |- | ||
| | | Total vol.||(8.5 μL)||408.0||408.0 | ||
|} | |} | ||
Revision as of 17:38, 14 May 2014
 Pc-TF Genomics
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05/14/14
RT-PCR: QC_plate, all cDNA batches
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