User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/14: Difference between revisions
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'''RT-PCR: QC_plate, all cDNA batches'''<br> | '''RT-PCR: QC_plate, all cDNA batches'''<br> | ||
* Run mCh, GAPD reactions on all cDNA batches, all cell lines: 16 total | * Summary: Run mCh, GAPD reactions on all cDNA batches, all cell lines: 16 total. Will be used to compare cDNA quality, PcTF expression, and generate reference values | ||
* Experiment file name: | * Experiment file name: | ||
* mCherry wells: 16x3 = 48 | * Target gene wells: mCherry wells: 16x3 = 48; GAPD wells: 16x3 = 48 | ||
* cDNA dilution: 1:1000 for all reactions | * cDNA dilution: 1:1000 for all reactions | ||
* Plate layout: Plate_layouts_Carly.xlsx/QC_plate | |||
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Primer/Probe Master mixes (2 tubes) | Target Gene Primer/Probe Master mixes (2 tubes) | ||
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| align="center" style="background:#f0f0f0;"|'''Reagent''' | | align="center" style="background:#f0f0f0;"|'''Reagent''' |
Revision as of 17:52, 14 May 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||
05/14/14
RT-PCR: QC_plate, all cDNA batches
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