User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/14

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  • RT-PCR: QC_plate, all cDNA batches

RT-PCR: QC_plate, all cDNA batches

  • Run mCh, GAPD reactions on all cDNA batches, all cell lines: 16 total
  • Will be used to compare cDNA quality, PcTF expression, and generate reference values
  • Experiment file name:
  • mCherry wells: 16x3 = 48
  • GAPD wells: 16x3 = 48

Primer/Probe Master mixes (2 tubes)

Reagent (Single well) Gene Target mCh Gene Target GAPD (x10)
2x LC480 Probes Master(7.5 μL)360.0360.0
20 μM Forward primer(0.3 μL)14.414.4 GAPD primers
20 μM Reverse primer(0.3 μL)14.40
10 μM UPL probe(0.3 μL)14.414.4 GAPD probe
PCR H2O(0.1 μL)4.819.2
Total vol.(8.5 μL)408.0408.0

Template Master Mixes (16 tubes)

Reagent (Single well) cDNA Template no-template control
diluted Batch# cDNA(2.0 μL)12.00
PCR H2O(4.5 μL)27.00.0
Total vol.(6.5 μL)39.00.0

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