User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/14: Difference between revisions
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| align="center" style="background:#f0f0f0;"|'''Verdict''' | | align="center" style="background:#f0f0f0;"|'''Verdict''' | ||
|- | |- | ||
| K562_E001 || A1/A4 || 36.416 || 30.876 || 2.15E-2 || | | K562_E001 || A1/A4 || 36.416 || 30.876 || 2.15E-2 || bad mCh | ||
|- | |- | ||
| K562_C001 || --- || 28.381 || 29.313 || 1.907 || --- | | K562_C001 || --- || 28.381 || 29.313 || 1.907 || --- | ||
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| K562_C001 || --- || 35.233 || 29.435 || 1.80E-2 || --- | | K562_C001 || --- || 35.233 || 29.435 || 1.80E-2 || --- | ||
|- | |- | ||
| K562_E003 || C1/C4 || | | K562_E003 || C1/C4 || 36.624 || 0 || invalid || not enough template | ||
|- | |- | ||
| K562_C003 || --- || | | K562_C003 || --- || 34.329 || 0 || invalid || not enough template | ||
|- | |- | ||
| SKNSH_E001 || D1/D4 || | | SKNSH_E001 || D1/D4 || 20.812 || 29.718 || 480.1 || good mCh expression | ||
|- | |- | ||
| SKNSH_C001 || | | SKNSH_C001 || --- || 33.691 || 30.259 || 9.27E-2 || good | ||
|- | |- | ||
| SKNSH_E002 || | | SKNSH_E002 || E1/E4 || 20.810 || 29.964 || 569.5 || good mCh expression | ||
|- | |- | ||
| SKNSH_C002 || | | SKNSH_C002 || --- || 32.599 || 29.739 || 0.1377 || good | ||
|- | |- | ||
| SKNSH_E003 || | | SKNSH_E003 || F1/F4 || 21.614 || 31.060 || 697.4 || good mCh expression | ||
|- | |- | ||
| SKNSH_C003 || | | SKNSH_C003 || --- || 36.165 || 30.564 || 2.06E-2 || good | ||
|- | |- | ||
| U2OS_E001 || | | U2OS_E001 || G1/G4 || 34.713 || 24.584 || 8.93E-4 || poor mCh signal, good GAPDH | ||
|- | |- | ||
| U2OS_C001 || | | U2OS_C001 || --- || 35.686 || 24.563 || 4.49E-4 || good GAPDH signal | ||
|- | |- | ||
| U2OS_E002 || | | U2OS_E002 || H1/H4 || 34.961 || 24.539 || 7.29E-4 || poor mCh signal, good GAPDH | ||
|- | |- | ||
| U2OS_C002 || | | U2OS_C002 || --- || 36.290 || 24.679 || 3.20E-4 || good GAPDH signal | ||
|} | |} | ||
Run whole plate again with 1:100 dilutions | Run whole plate again with 1:100 dilutions (10x more concentrated cDNA) | ||
BAR CHART<br> | BAR CHART<br> |
Revision as of 18:50, 16 May 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/14/14
RT-PCR: QC_plate, all cDNA batches
Target Gene Primer/Probe Master mixes (2 tubes)
Run whole plate again with 1:100 dilutions (10x more concentrated cDNA) BAR CHART
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