User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
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| <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | | <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | ||
|- | |- | ||
| 1. K562 1 || 0.975 || 2.017 || 780.25 || | | 1. K562 1 || 0.975 || 2.017 || 780.25 || 3.2 μL (2.5 μg) | ||
|- | |- | ||
| 2. K562 2 || 0.890 || 2.021 || 711.81 || | | 2. K562 2 || 0.890 || 2.021 || 711.81 || 3.5 μL (2.5 μg) | ||
|- | |- | ||
| 2. K562 3 || 0.856 || 2.028 || 684.53 || | | 2. K562 3 || 0.856 || 2.028 || 684.53 || 3.7 μL (2.5 μg) | ||
|- | |- | ||
| 2. K562 4 || 0.696 || 2.020 || 556.52 || | | 2. K562 4 || 0.696 || 2.020 || 556.52 || 4.5 μL (2.5 μg) | ||
|} | |} | ||
Revision as of 19:35, 4 June 2014
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06/04/14
cDNA synthesis: K562
oligo(dT) Primer annealing
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
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