User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
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'''cDNA synthesis mix''' | '''cDNA synthesis mix''' | ||
* Total reactions = | * Total reactions = 12 <!-- Replace N with the total number of reactions! --> | ||
* Samples: | * Samples: | ||
# K562 | # K562 1 (E004) | ||
# K562 1 | # K562 1 (E005) | ||
# K562 2 | # K562 1 (E006) | ||
# K562 2 | # K562 2 (C004) | ||
# K562 2 (C005) | |||
# K562 2 (C006) | |||
# K562 3 (E007) | |||
# K562 3 (E008) | |||
# K562 3 (E009) | |||
# K562 3 (C007) | |||
# K562 3 (C008) | |||
# K562 3 (C009) | |||
{| class="wikitable" border="0" cellspacing="5" | {| class="wikitable" border="0" cellspacing="5" | ||
|- | |- | ||
| <u>Reagent</u> || <u>Single rxn.</u> || Mix ( | | <u>Reagent</u> || <u>Single rxn.</u> || Mix (x12) | ||
|- | |- | ||
| 10x RT buffer || 2.0 || | | 10x RT buffer || 2.0 || 24.0 | ||
|- | |- | ||
| 25 mM MgCl<sub>2</sub> || 4.0 || | | 25 mM MgCl<sub>2</sub> || 4.0 || 48.0 | ||
|- | |- | ||
| 0.1 M DDT || 2.0 || | | 0.1 M DDT || 2.0 || 24.0 | ||
|- | |- | ||
| RNaseOUT || 1.0 || | | RNaseOUT || 1.0 || 12.0 | ||
|- | |- | ||
| SuperScript III RT || 1.0 || | | SuperScript III RT || 1.0 || 12.0 | ||
|- | |- | ||
| || 10.0 μL || | | || 10.0 μL || 120.0 μL | ||
|} | |} | ||
Revision as of 20:18, 4 June 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/04/14
cDNA synthesis: K562
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
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