User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
From OpenWetWare
Line 10: | Line 10: | ||
==06/04/14== | ==06/04/14== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* cDNA synthesis: K562 sets | * cDNA synthesis: K562 sets 4 - 9 | ||
---- | ---- | ||
'''cDNA synthesis: K562'''<br> | '''cDNA synthesis: K562'''<br> | ||
* Use Carly's RNA from -80 °C | * Use Carly's RNA from -80 °C | ||
* Assume that K562 1 | * Assume that K562 1 and 3 are +PcTF and K562 2 and 4 are controls (will find out when QC plate for mCh RT-PCR is run) | ||
* Will hold off on continuing U2OS for transiently transfected samples; will switch to KAH126 for further analyses | * Will hold off on continuing U2OS for transiently transfected samples; will switch to KAH126 for further analyses | ||
Revision as of 20:19, 4 June 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/04/14
cDNA synthesis: K562
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
|