User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
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* Conclusion: <font color="red">discard samples</font> | * Conclusion: <font color="red">discard samples</font> | ||
* Do a new RNA extraction from TRIzol samples | * Do a new RNA extraction from TRIzol samples | ||
'''RNA extraction''' | '''RNA extraction''' | ||
* Follow the TRIzol/ RNeasy protocol | * Follow the TRIzol/ RNeasy protocol | ||
* Use Carly's TRIzol samples K562 1, 2, 3, and 4 | * Use Carly's TRIzol samples K562 1, 2, 3, and 4 | ||
'''Measure RNA concentration'''<br> | '''Measure RNA concentration'''<br> | ||
{| class="wikitable" border="0" cellspacing="5" | {| class="wikitable" border="0" cellspacing="5" | ||
|- | |- | ||
| <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | | <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | ||
|- | |- | ||
| 1. K562 1 || | | 1. K562 1 || 0.975 || 2.017 || 780.25 || ### μL (2.5 μg) | ||
|- | |- | ||
| 2. K562 2 || | | 2. K562 2 || 0.890 || 2.021 || 711.81 || ### μL (2.5 μg) | ||
|- | |- | ||
| 2. K562 3 || | | 2. K562 3 || 0.856 || 2.028 || 684.53 || ### μL (2.5 μg) | ||
|- | |- | ||
| 2. K562 4 || | | 2. K562 4 || 0.696 || 2.020 || 556.52 || ### μL (2.5 μg) | ||
|} | |} | ||
Revision as of 19:33, 4 June 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
06/04/14
cDNA synthesis: K562
oligo(dT) Primer annealing
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
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