User:Karmella Haynes/Notebook/PcTF Genomics/2014/06/04: Difference between revisions
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| <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | | <u>Sample</u> || <u>OD 260</u> || <u>260/280</u> || <u>ng/μL</u> || <u>Vol. to use for cDNA synth.</u> | ||
|- | |- | ||
| 1. K562 1.1 || | | 1. K562 1.1 || -0.006 || 1.968 || -5.193 || n/a | ||
|- | |- | ||
| 2. K562 2.1 || | | 2. K562 2.1 || -0.003 || 1.6 || -2.724 || n/a | ||
|} | |} | ||
* Conclusion: discard samples | |||
* Do a new RNA extraction from TRIzol samples | |||
Revision as of 12:58, 4 June 2014
Pc-TF Genomics | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||
06/04/14
cDNA synthesis: K562
--> Incubate at 65°C/ 5 min. Immediately place on ice for 1 min.
--> Aliquot 10 μL of mix into 8-tube strip
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