User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/09: Difference between revisions
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Transfections | |||
* HEK293 grown in 2 mL DMEM/FBS (no pen-strep) | |||
* Removed 1 mL old medium | |||
* Carefully & slowly added 1 mL fresh medium (cells easily detach if not careful) | |||
* Added Lipo/DNA complexes drop-wise | |||
* Spun plates for 30 min (Seron's protocol - 'helps complexes to enter cells') | |||
* (Seron) placed cells into 37°C incubator | |||
Note: return to lab at 10 am tomorrow to change out medium | |||
Revision as of 17:09, 9 September 2014
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09/09/14
Lenti Transfections
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