User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/22: Difference between revisions

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(Autocreate 2014/09/22 Entry for User:Karmella_Haynes/Notebook/PcTF_Genomics)
 
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==mm/dd/yy==
==09/22/14==
<!-- Precede finished items with a checkmark &#x2713; -->
<!-- Precede finished items with a checkmark &#x2713; -->
* Line item 1
* 6-well plates for transductions


----
----
'''Line item 1'''<br>
'''Cultures for Transduction'''<br>
> Samples
# Plate 1: U2OS
# Plate 2: SK-N-SH
# Plate 3: K562 a (suspension)
# Plate 4: K562 b (suspension)


{| class="wikitable" border="0" cellspacing="3" <!-- Rxn. table -->
* Plated at ~40,000 cells per well
|-valign="top"
** Diluted 100% confluent 10 mL harvested cells as follows: 0.3 mL in 15 mL medium
| <u>Reagent</u> || <u>Volume</u>
* 2 mL media per well
|-
| reagent 1 || # μL
|-
| reagent 2 || #
|-
| reagent 3 || #
|-
| reagent 4 || #
|-
| dH<sub>2</sub>O || #
|-
| &nbsp; || # μL
|}
 
--> Reaction conditions


* For K562...
** a: made a 40k cell/ 2 mL / well plate
** b: made a 40k cell/ 1mL / well plate - transduction protocol calls for removal of 1mL medium prior to transduction. Set up this plate to avoid having to remove the 1 mL from suspended cells




Revision as of 06:22, 28 September 2014

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09/22/14

  • 6-well plates for transductions

Cultures for Transduction

  1. Plate 1: U2OS
  2. Plate 2: SK-N-SH
  3. Plate 3: K562 a (suspension)
  4. Plate 4: K562 b (suspension)
  • Plated at ~40,000 cells per well
    • Diluted 100% confluent 10 mL harvested cells as follows: 0.3 mL in 15 mL medium
  • 2 mL media per well
  • For K562...
    • a: made a 40k cell/ 2 mL / well plate
    • b: made a 40k cell/ 1mL / well plate - transduction protocol calls for removal of 1mL medium prior to transduction. Set up this plate to avoid having to remove the 1 mL from suspended cells



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