User:Karmella Haynes/Notebook/PcTF Genomics/2014/09/24

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09/24/14

  • Transduction Day 2: change media


Transduction Day 2

  • Check GFP controls
    • Plate 1: U2OS, ~90% GFP expression, at least ~two doubling
    • Plate 2: SK-N-SH, ~90% expression, slow cell growth
    • Plate 4: K562, no GFP expression. Did not continue next steps for this plate
  • Remove media
  • Add 2 ml/well fresh media with 1% Pen/strep
  • Grow cells at 37°C (overnight)



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